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用微计算机断层扫描法对无节段3D 组织学进行固定染色、全尺寸、毫米度试样的刚性嵌入

JoVE Journal
Bioingegneria

This content is Free Access.

JoVE Journal Bioingegneria

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

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用微计算机断层扫描法对无节段3D 组织学进行固定染色、全尺寸、毫米度试样的刚性嵌入

DOI:

10.3791/58293-v

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07:41 min

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October 17, 2018

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Alex Y. Lin², Yifu Ding^2,3, Daniel J. Vanselow², Spencer R. Katz^2,3, Maksim A. Yakovlev², Darin P. Clark, David Mandrell, Jean E. Copper², Damian B. van Rossum², Keith C. Cheng²

¹The Jake Gittlen Laboratories for Cancer Research,Penn State College of Medicine, ²Division of Experimental Pathology, Department of Pathology,Penn State College of Medicine, ³Medical Scientist Training Program,Penn State College of Medicine, ⁴Center for In Vivo Microscopy,Duke University Medical Center, ⁵KTM Research

Capitoli

00:04Titolo
01:05Fish Fixation
01:48Dehydration and Staining
02:24Infiltration
03:07Embedding
05:18Results: Successful Embedding Using Custom-designed Adapter
07:01Conclusion

Summary

Traduzione automatica

我们开发了协议, 并设计了一个定制装置, 以实现毫米级试样的嵌入。我们提出了样品制备程序, 重点是在丙烯酸树脂和聚酰亚胺管中嵌入, 以实现刚性固定化和长期储存标本, 用于审讯组织结构和细胞形态学的显微 CT。

Tags

Keywords: 3D Histology Micro-CT Whole Mount Embedding Staining Sample Preparation Zebrafish Fixed Specimens Rigid Embedding Long-term Storage High-throughput Phenotyping Toxicology Diagnostics

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