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Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

DOI:
10.3791/58299-v

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07:00 min

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October 08, 2018

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, , ,
1Faculty of Medicine and Life Sciences,University of Tampere, 2Oral and Maxillofacial Unit,Tampere University Hospital

Capitoli

  • 00:04Titolo
  • 00:37Culturing of Mycobacterium marinum and Preparation of Bacterial Solution for Infecting Adult Zebrafish
  • 02:10Experimental M. marinum Infection with Intraperitoneal Injection
  • 03:32Collection of Internal Organs
  • 04:36Results: Mycobacterium marinum Infection Modeling
  • 06:02Conclusion

Summary

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Here, we present a protocol to model human tuberculosis in an adult zebrafish using its natural pathogen Mycobacterium marinum. Extracted DNA and RNA from the internal organs of infected zebrafish can be used to reveal the total mycobacterial loads in the fish and the host's immune responses with qPCR.

Tags

Keywords: Mycobacterium MarinumTuberculosisZebrafishAdaptive Immune ResponsesPathogenesisNucleic AcidBacterial LoadsGene Expression7H10 Plate7H9 MediumADC EnrichmentPolysorbate 80GlycerolCell Culture FlaskInoculation LoopOD600PBSPhenol Red27 Gauge Needle30 Gauge Insulin NeedleRag Mutant7H10 Plates

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