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Developmental Biology

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JoVE Journal Developmental Biology
Analysis of Epididymal Protein Synthesis and Secretion
 

Analysis of Epididymal Protein Synthesis and Secretion

Article DOI: 10.3791/58308-v • 10:23 min • August 25th, 2018
August 25th, 2018 •
1,2, 3, 1,2, 2,4, 1,2
1Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, University of Newcastle, 2Hunter Medical Research Institute, 3Department of Physiology, Turku Center for Disease Modeling, Institute of Biomedicine, University of Turku, 4School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle

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Here, we report the immunofluorescence localization of dynamin to illustrate the protocols for the detection of proteins in paraffin-embedded mouse epididymal sections and those of an immortalized epididymal cell line (mECap18). We also describe the protocols for the isolation of secretory proteins from both epididymal fluid and conditioned cell media.

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Epididymal Protein Synthesis Epididymal Protein Secretion Sperm Maturation Storage Regulation Epididymal Isolation Technique Xylene Tissue Section Graded Ethynol Solutions Purified Water Antigen Retrieval Solution Microwave Heat-induced Antigen Retrieval Solutions PBS Liquid-repellent Slide Marker Pen Blocking Solution Primary Antibody
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