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高纯度凋亡体的检测与分离

JoVE Journal
Biochimica
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JoVE Journal Biochimica

Detection and Isolation of Apoptotic Bodies to High Purity

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高纯度凋亡体的检测与分离

DOI:

10.3791/58317-v

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12:17 min

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August 12, 2018

•

Thanh Kha Phan, Ivan KH Poon, Georgia K Atkin-Smith

¹Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science,La Trobe University

Capitoli

00:00Titolo
00:53Induction of Apoptosis
02:34Apoptotic Body Isolation using FACS
04:22ApoBD Isolation via Differential Centrifugation
06:02Flow Cytometry Gating Strategy
10:56Representative Result: Validation of Apoptosis
11:16Representative Result: Comparison of ApoBD-Enriched Sample Purity
11:47Conclusion

Summary

Traduzione automatica

利用流式细胞仪或差速离心的工作流来检测、定量和分离凋亡样本中的凋亡体, 以高纯度。

Tags

Apoptotic Bodies Extracellular Vesicles Hematosis Cell Clearance Intracellular Communication Apoptosis Induction UV Irradiation Apoptotic Morphologies Apoptotic Blebbing FACS Annexin V TOPO-3 Cell Strainer Cell Lines THB1 Monocytes

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