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Visualisere Node og Notochordal Plate i Gastrulating Mouse embryoer skanning elektronmikroskop og hele Mount Immunofluorescence
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1Graduate Program in Pharmacology and Experimental Therapeutics,University of Cologne, 2Department of Dermatology and Venereology,University Hospital of Cologne, 3Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD),University of Cologne, 4Department of General Ecology, Institute for Zoology, Biocenter Cologne,University of Cologne
Capitoli
- 00:04Titolo
- 00:59Scanning Electron Microscopy of the Mouse Embryonic Node
- 04:17Whole Mount Immunofluorescence of the Mouse Node and Notochordal Plate
- 08:06Results: Strip1 Mutant Embryos Display Irregular Nodes and Abnormal F-Actin Organization in the Axial Mesoderm
- 08:46Conclusion
Noden og notochordal plate er forbigående signalering arrangørene utvikle mouse embryoer som kan visualiseres ved hjelp av flere teknikker. Her vi beskrive i detalj hvordan du utfører to teknikker for å studere struktur og morphogenesis: 1) skanning elektronmikroskop (SEM); og 2) hele mount immunofluorescence (WMIF).
