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果蝇S2R+ 细胞折叠-肠信号通路检测非肌球蛋白 II . 收缩力的细胞基础研究
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells
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果蝇S2R+ 细胞折叠-肠信号通路检测非肌球蛋白 II . 收缩力的细胞基础研究

DOI:
10.3791/58325-v

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07:15 min

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August 19, 2018

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, , , , , , ,
1Department of Biology,The University of North Carolina at Chapel Hill, 2Department of Biology,Reed College, 3Carolina Center for Genome Sciences,The University of North Carolina at Chapel Hill, 4Lineberger Comprehensive Cancer Center,The University of North Carolina at Chapel Hill

Capitoli

  • 00:04Titolo
  • 01:10Preparation of Concanavalin-A-coated Glass-bottomed Dishes and the Plating of S2R+ Cells
  • 02:25Efficacy Test of the Fog-conditioned Medium and the Cellular Contractility Assay
  • 03:16Fixing and Staining of Constricted S2R+ Cells
  • 05:18Imaging and Quantification of the Cellular Contractility Assay
  • 05:41Results: Post-treatment with Fog-Myc, RLC Forms Rings Indicative of Non-Muscle Myosin II Constriction
  • 06:40Conclusion

Summary

Traduzione automatica

Traduzione automatica

这里我们描述了一种使用果蝇S2R+ 细胞的收缩力测定方法。外源配体, 折叠肠 (雾) 的应用, 导致了雾信号通路和细胞收缩力的活化。该方法可用于研究雾信号通路中细胞收缩蛋白的调节。

Tags

Keywords: Non-muscle Myosin IIContractilityFolded-gastrulation Signaling PathwayDrosophila S2R+ CellsCell-based AssayCell DensityCell MorphologyCell Shape ChangeMorphogenesisDevelopmentGene DiscoveryRNAi Depletion

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