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Immunology and Infection

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JoVE Journal Immunology and Infection
Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding
 

Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

Article DOI: 10.3791/58341-v • 09:12 min • November 14th, 2018
November 14th, 2018 •
1, 1,2,3, 1, 1, 1, 4, 1,2
1Department of Psychiatry, Columbia University, 2Department of Pathology & Cell Biology, Columbia University Medical Center, 3Department of Psychiatry, Division of Molecular Imaging and Neuropathology, New York State Psychiatric Institute, 4EB Sciences

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Here, we present a protocol to isolate brain nuclei in the neonatal rat brain in conjunction with first colostrum feeding. This technique allows the study of nutrient insufficiency stress in the brain as modulated by enterocyte signaling.

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Cellular Stress Inflammation Oxytocin-secreting Brain Nuclei Neonatal Rat Colostrum Feeding Milk Suckling Stress Signaling Brain Tissue Analysis Antagonist Ex-vivo Nuclei Punches Brain Mold Razor Blade Tissue Slices Petri Dish Artificial Cerebral Spinal Fluid Glucose Incubate Orbital Shaker Brain Atlas Anatomic Landmarks
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