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Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm

Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm

DOI:

10.3791/58347-v

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08:42 min

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October 04, 2018

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Dante J. Heredia, Grant W. Hennig, Thomas W. Gould

¹Department of Physiology and Cell Biology, School of Medicine,University of Nevada, ²Department of Pharmacology, Larner College of Medicine,University of Vermont

Capitoli

00:04Titolo
00:27Preparation of the Diaphragms and Phrenic Nerves from Transgenic Mice
01:27Stimulation and Recording of the Muscle Action Potentials
04:49Imaging of the Fluorescence of the Sample
06:33Results: Nerve Stimulation-induced Ca²⁺ Responses in Populations of Schwann Cells or Muscle Cells of the Neonatal Mouse Diaphragm
08:03Conclusion

Summary

Traduzione automatica

Here we present a protocol to image calcium signaling in populations of individual cell types at the murine neuromuscular junction.

Tags

Keywords: Ex Vivo Imaging Calcium Signaling Tripartite Synapse Neuromuscular Phrenic Nerve Diaphragm Nerve Stimulation Muscle Cells Schwann Cells Transgenic Mice Krebs-Ringer Solution Alpha Bungarotoxin Suction Electrode BHC Mu-conotoxin

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