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磷流式细胞仪与荧光细胞条形码用于单细胞信号分析和生物标志物发现

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Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery

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磷流式细胞仪与荧光细胞条形码用于单细胞信号分析和生物标志物发现

DOI:

10.3791/58386-v

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08:38 min

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October 04, 2018

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Sigrid S. Skånland²

¹Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership,University of Oslo, ²K. G. Jebsen Centre for B cell malignancies and K. G. Jebsen Centre for Cancer Immunotherapy,University of Oslo

Capitoli

00:04Titolo
00:32Preparation, Stimulation, and Fixation of Cells
01:50Fluorescent Cell Barcoding
03:30Cell Permeabilization and Intracellular Antigen Staining
06:12Results: Phospho Flow Cytometry Analysis with Fluorescent Cell Barcoding
08:02Conclusion

Summary

Traduzione automatica

在这里, 提出了一种中-高通量分析在细胞级蛋白质磷酸化事件的协议。磷流式细胞术是表征信号畸变、识别和验证生物标志物、评估药效学的有力手段。

Tags

Keywords: Phospho Flow Cytometry Fluorescent Cell Barcoding Single Cell Signaling Analysis Biomarker Discovery Cell Biology Immunology Signaling Pathways Stimuli Drugs High Throughput RPMI 16/40 Medium Fixed Buffer Anti-IgM PBS Barcoding Reagents Perm Buffer Compensation Control

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