/

/

荷尔斯坦奶牛肝线粒体耗氧量和质子泄漏动力学评价线粒体呼吸

JoVE Journal
Biochimica

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Biochimica

Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle

Please note that all translations are automatically generated. Click here for the English version.

荷尔斯坦奶牛肝线粒体耗氧量和质子泄漏动力学评价线粒体呼吸

DOI:

10.3791/58387-v

•

08:29 min

•

November 30, 2018

•

Heidi A. Rossow, Gabriela Acetoze, John Champagne, Jon J. Ramsey

¹Veterinary Medicine Teaching and Research Center,University of California Davis, ²Archer Daniels Midland, ³Department of Molecular Biosciences,University of California Davis

Capitoli

00:04Titolo
00:54Isolating Mitochondria from Dairy Cow Liver
02:46Measuring Mitochondrial Oxygen Consumption
04:33Measuring Mitochondrial Membrane Potential (MMP) and Proton Motive Force (PMF)
06:42Results: Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics
07:45Conclusion

Summary

Traduzione automatica

在这里, 我们分享测量线粒体耗氧量的方法, 营养能量学的一个决定性概念, 和质子泄漏, 这是线粒体产生 atp 效率低下的主要原因。这些结果可以占营养利用损失能量的 30%, 以帮助评估线粒体功能。

Tags

Keywords: Mitochondrial Oxygen Consumption Proton Leak Kinetics Mitochondrial Respiration Dairy Cattle Energetics Mitochondrial Isolation Liver Sample Homogenization Centrifugation Protein Concentration Oxygen Consumption Assay Respiration Chamber Oxygraph System

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

使用脚手架脂质体重构脂质近端蛋白质 - 蛋白质相互作用<em>体外</em

线粒体钙在分离线粒体和培养细胞中的流入分析

用最小组织量测定果蝇透纤维中线粒体耗氧量

全飞头段耗氧率的测定与解释

测量来自母猪后代的先前冷冻心脏组织中的线粒体电子转移复合物：评估运动诱导的线粒体生物能量变化的模型

测量哺乳动物线粒体功能的氧非依赖性测定

基于图像的高通量线粒体DNA合成和分布定量

一种分离线粒体接触位点的改进方法

使用高分辨率呼吸测量法分析果蝇黑腹果蝇 PINK1^B9-Null 突变体的线粒体功能

提交软骨囊泡的分离和分析以鉴定线粒体接触位点蛋白

Read Article