/
/
Isolatie van volwassen ruggenmerg kernen voor Massively Parallel Single-nucleus RNA Sequencing
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Spinal Circuits and Plasticity Unit,National Institute of Neurological Disorders and Stroke, 2Bioinformatics Section, Information Technology Program,National Institute of Neurological Disorders and Stroke, 3Laboratory of Cochlear Development,National Institute on Deafness and Other Communication Disorders, 4Single Cell Analysis Facility,Frederick National Laboratory
Capitoli
- 00:04Titolo
- 00:43Preparation of the Spinal Cord
- 02:03Detergent-mechanical Cell Lysis
- 03:25Homogenization and Sucrose Density Gradient
- 05:11Results: Representative Results of Nuclei Isolated from the Adult Mouse Spinal Cord
- 05:51Conclusion
Hier presenteren we een protocol om snel isoleren kwalitatief hoogwaardige kernen uit de verse of bevroren weefsel voor downstream massaal parallelle RNA sequencing. Wij omvatten wasmiddel-mechanische en hypotone-mechanische weefsel verstoring en cel lysis van de opties, die beide kunnen worden gebruikt voor isolatie van kernen.
