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Inducerbar og Reversible dominerende-negative (DN) Protein hæmning
JoVE Journal
Genetica
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JoVE Journal Genetica
Inducible and Reversible Dominant-negative (DN) Protein Inhibition
DOI:

08:35 min

January 07, 2019

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Capitoli

  • 00:04Titolo
  • 00:58In Vitro Testing of the TetO-DN-CB-myc6-Rb1 Transgene in NIH3T3 Cells
  • 02:19In Vitro Testing of the TetO-DN-CB-myc6-Rb1 Transgene in HEI-OC1 Cells
  • 05:37Generation of CBRb+/ROSA-CAG-rtTA+ (CBRb) Transgenic Mice and In Vivo Testing of the DN-CBRb Approach
  • 06:09Results: Confirmation of Efficiency of TetO-DN-CB-myc6-Rb1 Construct
  • 07:50Conclusion

Summary

Traduzione automatica

Her præsenterer vi en protokol for at udvikle en dominerende negativ inducerbar system, som kan enhver protein betinget inaktiveret ved reversibelt overekspression en dominerende negativ mutant version af det.

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