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Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons

Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons

DOI:
10.3791/58421-v

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10:50 min

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November 02, 2018

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, , ,
1UNC Neuroscience Center, 2UNC/NC State Joint Department of Biomedical Engineering,UNC, 3Xona Microfluidics, LLC

Capitoli

  • 00:04Titolo
  • 01:27Preparation and Coating of the Multicompartment Chips
  • 03:57Seeding Neurons into the Multicompartment Chips
  • 05:03Fluidic Isolation of the Axonal Compartment within the Chip
  • 05:49Performing Axotomy Within the Chip
  • 06:36Fluorescence Immunostaining within the Chip
  • 09:03Results: Neuronal Growth Comparison within Multicompartment PDMS Devices and Plastic Chips
  • 10:21Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes the use of plastic chips to culture and compartmentalize primary murine neurons. These chips are preassembled, user-friendly, and compatible with high-resolution, live, and fluorescence imaging. This protocol describes how to plate rat hippocampal neurons within these chips and perform fluidic isolation, axotomy and immunostaining.

Tags

Keywords: Microfluidic DevicesCompartmentalized CulturePrimary NeuronsPreassembled Plastic ChipsNeuroscienzeAxon ExtensionSynaptic RemodelingAlzheimer’s DiseaseStem CellsPrecoding SolutionPBS Wash

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