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预组装塑料微流体芯片在原生小鼠神经元分割中的应用
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons
DOI:

10:50 min

November 02, 2018

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Capitoli

  • 00:04Titolo
  • 01:27Preparation and Coating of the Multicompartment Chips
  • 03:57Seeding Neurons into the Multicompartment Chips
  • 05:03Fluidic Isolation of the Axonal Compartment within the Chip
  • 05:49Performing Axotomy Within the Chip
  • 06:36Fluorescence Immunostaining within the Chip
  • 09:03Results: Neuronal Growth Comparison within Multicompartment PDMS Devices and Plastic Chips
  • 10:21Conclusion

Summary

Traduzione automatica

该协议描述了使用塑料芯片培养和分割主要小鼠神经元。这些芯片是预装的, 用户友好的, 并与高分辨率, 活的和荧光成像兼容。该方案描述了如何在这些芯片内对大鼠海马神经元进行平板治疗, 并进行流控分离、轴切和免疫染色。

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