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Brug af formonterede plast mikrofluid Chips for Compartmentalizing primære Murine neuroner
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Capitoli
- 00:04Titolo
- 01:27Preparation and Coating of the Multicompartment Chips
- 03:57Seeding Neurons into the Multicompartment Chips
- 05:03Fluidic Isolation of the Axonal Compartment within the Chip
- 05:49Performing Axotomy Within the Chip
- 06:36Fluorescence Immunostaining within the Chip
- 09:03Results: Neuronal Growth Comparison within Multicompartment PDMS Devices and Plastic Chips
- 10:21Conclusion
Denne protokol beskriver brugen af plastik chips til kultur og inddeler primære murine neuroner. Disse chips er hovedmeningen, brugervenlig og kompatibel med høj opløsning, lever, og fluorescens billeddannelse. Denne protokol beskriver, hvordan plade rotte hippocampus neuroner i disse chips og udføre fluidic isolering, axotomy og immunfarvning.
