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cd4+ t 细胞的分离及6色流式细胞仪分析外周血中循环 t-滤泡 (ctfh) 细胞亚群
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Biologia
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JoVE Journal Biologia
Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry
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cd4+ t 细胞的分离及6色流式细胞仪分析外周血中循环 t-滤泡 (ctfh) 细胞亚群

DOI:
10.3791/58431-v

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07:39 min

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January 07, 2019

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, , , ,
1Leicester Cancer Research Centre and Ernest and Helen Scott Haematology Research Institute,University of Leicester, 2MRC Toxicology Unit,University of Leicester

Capitoli

  • 00:04Titolo
  • 00:43CD4+ T Cell Peripheral Whole Blood Isolation and Flow Cytometric Analysis
  • 04:45Data Analysis
  • 06:07Results: Representative Circulating T-follicular Helper (cTfh) Cell Subset Analyses
  • 06:53Conclusion

Summary

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本文介绍了一种从人外周血中分离和表征 cd4 + t 细胞亚群的方法。采用流式细胞仪对纯化的 cd4+ t 细胞进行分析, 以确定 t-滤泡辅助细胞亚群的比例。

Tags

CD4+ T-cellsT-follicular Helper (Tfh) CellsCirculating T-follicular Helper (cTfh) CellsFlow CytometryBiomarkerAutoimmune DiseaseB-cell Non-Hodgkin’s LymphomaPeripheral BloodCell IsolationCell Subsets

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