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Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart
 

Isolation, Characterization, and Differentiation of Cardiac Stem Cells from the Adult Mouse Heart

Article DOI: 10.3791/58448-v • 11:45 min • January 7th, 2019
January 7th, 2019 •
1, 1,2
1Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, 2Department of Anesthesiology, University of Nebraska Medical Center

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The overall goal of this article is to standardize the protocol for the isolation, characterization, and differentiation of cardiac stem cells (CSCs) from the adult mouse heart. Here, we describe a density gradient centrifugation method to isolate murine CSCs and elaborated methods for CSC culture, proliferation, and differentiation into cardiomyocytes.

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Keywords: Cardiac Stem Cells CSCs Adult Mouse Heart Isolation Characterization Differentiation Cardiovascular Disease Heart Failure Cardiac Stem Cell Therapy Cost-effective Ham's Balanced Salt Solution Collagenase II Tissue Digestion Cell Suspension
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