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用两相循环流系统实时成像和定量真菌生物膜的研究
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Real-time Imaging and Quantification of Fungal Biofilm Development Using a Two-Phase Recirculating Flow System
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用两相循环流系统实时成像和定量真菌生物膜的研究

DOI:
10.3791/58457-v

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06:16 min

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October 18, 2018

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,
1Department of Oral Biology,University at Buffalo

Capitoli

  • 00:04Titolo
  • 00:26Preparation of the Flow Apparatus
  • 02:44Imaging with the Flow Apparatus
  • 05:16Results: Candida albicans Biofilm Formation Under Fluid Flow
  • 05:58Conclusion

Summary

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介绍了一种在水流下实时成像真菌生物膜形成的流动装置的组装、操作和清洗。我们还提供并讨论了用于获取图像的定量算法。

Tags

Real-time ImagingFungal BiofilmTwo-phase Recirculating Flow SystemFluid FlowBiofilm Growth And DevelopmentFlow ApparatusBubble TrapTemperature ProbeFilterGrowth FlaskYPD MediaPumpSlide AdapterMicroscope

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