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多平行板流室对剪切应力暴露的内皮细胞基因表达分析
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Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers
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多平行板流室对剪切应力暴露的内皮细胞基因表达分析

DOI:
10.3791/58478-v

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08:50 min

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October 21, 2018

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, , , , ,
1Institute of Medical Science,University of Toronto, 2Keenan Research Centre in the Li Ka Shing Knowledge Institute,St. Michael’s Hospital, 3Department of Laboratory Medicine and Pathobiology,University of Toronto, 4Department of Medicine,University of Toronto

Capitoli

  • 00:04Titolo
  • 00:55Assay Setup
  • 04:20Flow Assay
  • 05:18Cell Harvest and RNA Extraction
  • 06:48Results: Representative KLF2 Loss-of-Function Analysis in Human Endothelial Cells in Response to Shear Stress
  • 08:06Conclusion

Summary

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本文介绍了流体剪切应力作用下内皮细胞培养和基因表达分析的工作流程。包括一个物理安排, 可同时在受控环境中同时外壳和监测多个流室, 并使用外源参考 RNA 进行定量 PCR。

Tags

Endothelial CellsShear StressParallel-plate Flow ChambersGene Expression AnalysisAtherosclerosisLaminar FlowCell CultureFibronectinCO2 IncubatorBubble TrapCell SeedingTubingStopcock

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