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Novel Methods for Intranasal Administration Under Inhalation Anesthesia to Evaluate Nose-to-Brain Drug Delivery
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JoVE Journal Medicina
Novel Methods for Intranasal Administration Under Inhalation Anesthesia to Evaluate Nose-to-Brain Drug Delivery

Novel Methods for Intranasal Administration Under Inhalation Anesthesia to Evaluate Nose-to-Brain Drug Delivery

DOI:
10.3791/58485-v

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05:44 min

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November 14, 2018

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, , ,
1Laboratory of Pharmaceutics, School of Pharmacy,Nihon University

Capitoli

  • 00:04Titolo
  • 01:04Intranasal Drug Administration
  • 03:11Radio-Labeled Water-Soluble Macromolecules ([14C]-inulin) Quantification
  • 04:32Results: Comparison of Brain [14C]-Inulin Levels After Two Different Methods of Intranasal Administration
  • 05:17Conclusion

Summary

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Here, we describe two novel methods of stable intranasal administration under inhalation anesthesia with minimal physical stress for experimental animals. We also describe a method for quantitative evaluation of drug distribution levels in the brain via the nose-to-brain pathway using radiolabeled [14C]-inulin as a model substrate of water-soluble macromolecules.

Tags

Intranasal AdministrationNose-to-brain Drug DeliveryInhalation AnesthesiaCarbon-14 InulinReverse CannulationEsophageal CannulaProgrammable Microsyringe PumpBlood-brain BarrierPharmacokineticsPharmacologyCentral Nervous System DiseasesDrug Delivery Technologies

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