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通过 iscat 显微镜对活细胞分泌的单一蛋白质进行无标签成像
JoVE Journal
Ingegneria
This content is Free Access.
JoVE Journal Ingegneria
Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy
DOI:

10:55 min

November 20, 2018

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Capitoli

  • 00:04Titolo
  • 01:18Building the Interferometric Scattering (iSCAT) Microscope
  • 06:48iSCAT Microscopy of Secreting Cells
  • 08:53Results: Label-Free Detection of Proteins Secreted by a Single Cell
  • 09:36Conclusion

Summary

Traduzione automatica

我们提出了一种实时光学检测单个未标记蛋白质的协议, 因为它们是从活细胞分泌的。这是基于干涉散射 (iscat) 显微镜, 可应用于各种不同的生物系统和配置。

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