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全完整型小鼠软骨外种机械负荷对软骨细胞损伤的实时可视化与分析
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Bioingegneria
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JoVE Journal Bioingegneria
Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants
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全完整型小鼠软骨外种机械负荷对软骨细胞损伤的实时可视化与分析

DOI:
10.3791/58487-v

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08:42 min

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January 07, 2019

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, ,
1Department of Biomedical Engineering,University of Rochester

Capitoli

  • 00:04Titolo
  • 00:44Distal Femur Collection
  • 03:08Proximal Humerus Collection
  • 04:45Live (Calcein AM)/Dead (Propidium Iodide) Staining and Mechanical Testing
  • 07:29Results: Representative Femoral and Humeral Articular Surface Injury Analyses
  • 08:03Conclusion

Summary

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我们提出了一种方法来评估细胞损伤死亡的空间程度后, 完整的小鼠关节关节应用受控机械负荷或冲击。该方法可用于研究骨关节炎、遗传因素和不同的加载方案如何影响原位软骨细胞的脆弱性。

Tags

Real-time VisualizationChondrocyte InjuryMechanical LoadingMurine Cartilage ExplantsArticular ChondrocytesMouse ModelFemur DissectionKnee JointCartilage DegenerationMechanical Injury

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