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免疫荧光共聚焦显微镜对单纯疱疹病毒1蛋白核细胞质转移的时间分析

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Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

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免疫荧光共聚焦显微镜对单纯疱疹病毒1蛋白核细胞质转移的时间分析

DOI:

10.3791/58504-v

•

06:40 min

•

November 04, 2018

•

Subodh Kumar Samrat, Haidong Gu

¹Department of Biological Sciences,Wayne State University

Capitoli

00:04Titolo
00:31Cell Seeding, Virus Infection, and Immunofluorescent Labeling
03:12Confocal Imaging and Nuclear vs. Cytoplasmic Distribution Analysis
05:12Results: Representative Infected Cell Protein 0 (ICP0) Distribution Analyses
05:56Conclusion

Summary

Traduzione automatica

icp0 在1型单纯疱疹病毒感染期间经历核细胞质转移。这个事件的分子机制尚不清楚。本文介绍了共聚焦显微镜作为量化1型单纯疱疹病毒感染 icp0 运动的工具, 为定量分析 icp0 在未来的机械研究中的移位奠定了基础。

Tags

Temporal Analysis Nuclear-to-cytoplasmic Translocation Herpes Simplex Virus 1 Protein Immunofluorescent Confocal Microscopy Protein Trafficking Light Imaging Virus Infection Immunofluorescent Staining Paraformaldehyde Permeabilization Blocking Buffer Primary Antibody Secondary Antibody DAPI

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