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Endotoxin Activity Assay for the Detection of Whole Blood Endotoxemia in Critically Ill Patients
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Endotoxin Activity Assay for the Detection of Whole Blood Endotoxemia in Critically Ill Patients

Endotoxin Activity Assay for the Detection of Whole Blood Endotoxemia in Critically Ill Patients

DOI:
10.3791/58507-v

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06:28 min

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June 24, 2019

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, , , , ,
1Department of Anesthesia and Critical Care,Niguarda Hospital, 2University of Milan-Bicocca Medical School

Capitoli

  • 00:04Titolo
  • 01:02Laboratory Equipment and Assay Kit Contents
  • 02:21Endotoxin Activity Assay
  • 04:51Results: Results of the Endotoxin Activity Assay
  • 05:41Conclusion

Summary

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We hereby present a protocol to measure at the bedside the endotoxin activity of human whole blood samples. The Endotoxin Activity assay is a simple test to perform and may be a useful biomarker in critically ill patients with sepsis.

Tags

Keywords: EndotoxinSepsisGram-negative BacteriaEndotoxemiaEndotoxin Activity AssayBiomarkerSeptic ShockNeutrophilsOxidative BurstLPSEDTAChemiluminometerIncubator Shaker

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