Rivista
/
Neuroscienze
/
Simultaneous Cryosectioning of Multiple Rodent Brains
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Simultaneous Cryosectioning of Multiple Rodent Brains

Simultaneous Cryosectioning of Multiple Rodent Brains

DOI:
10.3791/58513-v

•

06:37 min

•

September 18, 2018

•

, , , ,
1Department of Child Health,University of Arizona College of Medicine, 2Department of Biological Sciences,University of Bath, 3BARROW Neurological Institute at Phoenix Children’s Hospital, 4Phoenix Veteran Affairs Healthcare System, 5Department of Basic Medical Sciences,University of Arizona College of Medicine

Capitoli

  • 00:04Titolo
  • 00:44Cryoprotection of Perfused Brains and Brain Freezing
  • 03:18Preparing the Megabrain for Sectioning on the Cryostat
  • 04:14Cryosectioning the Megabrain
  • 05:24Results: Freezing and Sectioning Brain Tissue from Multiple Animals with Megabrain
  • 05:54Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present a protocol to freeze and section brain tissue from multiple animals as a timesaving alternative to processing single brains. This reduces staining variability during immunohistochemistry and reduces time cryosectioning and imaging.

Tags

Simultaneous CryosectioningMultiple Rodent BrainsImmunohistochemistryTime-saving MethodCryosectionEmbedding MoldIsopentaneOptimal Cutting TemperatureOCT CompoundTranscardial PerfusionParaformaldehydeSucrose SolutionCerebellumOlfactory BulbsBrain Hemispheres

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Simultaneous fMRI and Electrophysiology in the Rodent Brain

Ex vivo Culturing of Whole, Developing Drosophila Brains

Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex

Simultaneous Electrophysiological Recording and Micro-injections of Inhibitory Agents in the Rodent Brain

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains

Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains

Free-floating Immunostaining of Mouse Brains

Automated Multimodal Stimulation and Simultaneous Neuronal Recording from Multiple Small Organisms

Author Spotlight: High-Resolution Imaging of Mouse Neonate Brains – A Micro-CT Protocol with Lugol's Solution Contrast Agent

Read Article