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Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT

Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT

DOI:
10.3791/58528-v

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07:33 min

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November 27, 2019

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,
1Department of Clinical Sciences, Division of Infection Medicine,Lund University

Capitoli

  • 00:04Titolo
  • 00:34Analysis and Quantification of Neutrophil Extracellular Traps (NETs)
  • 02:19Segmentation of Cells in the DNA Channel and the NET Channel
  • 03:41Single-cell Analysis of Identifiable Properties
  • 04:19Comparison of Cell Properties to Identify NET-forming Cells
  • 05:23Result Outputs Demeanor
  • 06:16Results: Analysis of NET Formation
  • 07:07Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present a protocol for generating neutrophil extracellular traps (NETs) and operating NETQUANT, a fully automatic software option for quantification of NETs in immunofluorescence images.

Tags

NETQUANTAutomated Image AnalysisNeutrophil Extracellular Traps (NETs)Immunofluorescence ImagingImage SegmentationAdaptive SegmentationDNA ChannelNET ChannelSample TypesBatch ProcessingImage MetadataZenodoNordonfelt Lab

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