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Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
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Biologia
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JoVE Journal Biologia
Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

DOI:
10.3791/58545-v

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07:39 min

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June 04, 2019

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, , ,
1Biological Sciences Department Hunter College,City University of New York, 2Program in Molecular, Cellular, and Developmental Biology,Graduate Center, City University of New York

Capitoli

  • 00:04Titolo
  • 00:51Molecular Beacon (MB) Nurse Cell Microinjection
  • 02:38Tracking and Colocalization Analyses and Video File Preparation
  • 05:58Results: Representative MB Visualization and Tracking
  • 07:15Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present a protocol for the visualization, detection, analysis and tracking of endogenous mRNA trafficking in live Drosophila melanogaster egg chamber using molecular beacons, spinning disc confocal microscopy, and open-source analysis software.

Tags

Keywords: MRNADrosophila MelanogasterEgg ChambersRNA LocalizationLive ImagingMolecular BeaconsMicroinjectionRNA BiologyReal-time VisualizationRNA TraffickingTherapeutic TargetsZebrafishCell CultureMicroscopyImage Analysis

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