Rivista
/
Medicina
/
In Vitro Method to Control Concentrations of Halogenated Gases in Cultured Alveolar Epithelial Cells
JoVE Journal
Medicina
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Medicina
In Vitro Method to Control Concentrations of Halogenated Gases in Cultured Alveolar Epithelial Cells

In Vitro Method to Control Concentrations of Halogenated Gases in Cultured Alveolar Epithelial Cells

DOI:
10.3791/58554-v

•

04:56 min

•

October 23, 2018

•

, , , , , , , , , , , , ,
1Department of Perioperative Medicine,CHU Clermont-Ferrand, 2Centre National de la Recherche Scientifique Unité Mixte de Recherche (CNRS UMR) 6293, Institut National de la Santé et de la Recherche Médicale (INSERM) U1103, Laboratoire de Génétique, Reproduction et Développement (GReD),Université Clermont Auvergne, 3Department of Pharmacology,CHU Clermont-Ferrand, 4Nurse Anesthetist School,CHU Clermont-Ferrand

Capitoli

  • 00:04Titolo
  • 00:40Preparation of an Air-tight Chamber
  • 01:57Expose Alveolar Epithelial Cells to Halogenated Agents
  • 03:13Results: Concentration of Sevoflurane and Isoflurane Over Time
  • 03:56Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe an easy protocol specifically designed to reach precise and controlled concentrations of sevoflurane or isoflurane in vitro in order to improve our understanding of mechanisms involved in the epithelial lung injury and to test novel therapies for acute respiratory distress syndrome.

Tags

Keywords: In VitroHalogenated GasesAlveolar Epithelial CellsLung InjuryAcute Respiratory Distress SyndromeAirtight ChamberGas FlowCarbon DioxideSevofluraneIsoflurane

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/58554?article_type=v

A Simple Method of Mouse Lung Intubation
check_url/it/58554?article_type=v

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro
check_url/it/58554?article_type=v

In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells
check_url/it/58554?article_type=v

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
check_url/it/58554?article_type=v

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells
check_url/it/58554?article_type=v

Studying the Role of Alveolar Macrophages in Breast Cancer Metastasis
check_url/it/58554?article_type=v

Forskolin-induced Swelling in Intestinal Organoids: An In Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients
check_url/it/58554?article_type=v

A Method for Quantifying Upper Limb Performance in Daily Life Using Accelerometers
check_url/it/58554?article_type=v

Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing
check_url/it/58554?article_type=v

Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing

Read Article