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微胶质细胞抗体介导的陶氏清除研究
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia
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微胶质细胞抗体介导的陶氏清除研究

DOI:
10.3791/58576-v

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07:18 min

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November 09, 2018

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, , , , ,
1Neuroscience Discovery,Janssen Pharmaceutical Company of Johnson & Johnson, Belgium, 2Department of Hematopoiesis,Sanquin Research and Landsteiner Laboratory, The Netherlands, 3Janssen Prevention Center,Janssen Pharmaceutical Company of Johnson & Johnson, The Netherlands, 4Neuroscience Discovery,Janssen Pharmaceutical Company of Johnson & Johnson, Pennsylvania

Capitoli

  • 00:04Titolo
  • 00:37Uptake Assay with Fluorescence-activated Cell Sorting (FACS) Read-out
  • 04:33FACS Analysis
  • 05:41Results: Antibody-mediated Increase of Tau Uptake in BV-2 Cells
  • 06:45Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们描述了一种基于细胞的检测方法, 以定量评估小胶质细胞对 tau 的吸收, 目的是创建一个研究工具, 以更好地描述抗 tau 抗体的作用机制。

Tags

Keywords: Cell-based AssayAntibody-mediated Tau ClearanceMicrogliaAlzheimer’s DiseasePH Dye-Tau AggregatesAnti-Tau AntibodiesBV-2 CellsCell CultureTrypsin EDTACentrifugation

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