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Biocytin herstel en 3D-reconstructies van gevulde Hippocampal CA2 interneuronen
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
Biocytin Recovery and 3D Reconstructions of Filled Hippocampal CA2 Interneurons
DOI:

11:21 min

November 20, 2018

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Capitoli

  • 00:04Titolo
  • 00:26Tissue Preparation and Staining for Fluorescence Microscopy and 3D Neuronal Reconstruction
  • 04:08Histological Detection of Biotin-filled Neurons
  • 08:033D Neuronal Reconstructions
  • 10:12Results: Reconstruction of a Basket Cell Recorded and Filled in the Hippocampal CA2 Region and Morphometry Analyses
  • 10:57Conclusion

Summary

Traduzione automatica

Het protocol hier geschetste beschrijft de immunofluorescentie analyse, biocytin herstel en kwalitatief hoogwaardige reconstructies van hippocampal CA2 interneuronen na de intracellulaire elektrofysiologische opnames in vitro waardoor neuronale karakterisering en uiteindelijk prima neuronale anatomie worden bestudeerd.

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