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光漂白可实现前列腺素球菌中 ftsz 环的超分辨率成像
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus
DOI:

10:09 min

November 06, 2018

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Capitoli

  • 00:04Titolo
  • 00:43Sample Preparation and Fixation
  • 01:44Precoating of the Coverslip with Polystyrene Beads and Coating of Poly-L-lysine onto the Bead-coated Coverslip
  • 03:08Immobilization of Cells on the Coverslip and Permeabilization of Cyanobacterial Cells
  • 04:23Photobleaching of the Chlorophyll Pigments in a Blocking Step
  • 04:51Antibody Binding
  • 06:07Image Acquisition of STORM Data
  • 07:51Results: Super-resolution Imaging of the FtsZ Ringin in the Cyanobacterium Prochlorococcus
  • 09:42Conclusion

Summary

Traduzione automatica

在这里, 我们描述了一种光漂白方法, 以减少蓝藻的自体荧光。光漂白后, 采用随机光学重建显微镜获得蓝藻 ftsz 环的三维超分辨率图像。

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