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食品和环境样品中沙门氏菌的准总体基因体分析
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Biologia
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JoVE Journal Biologia
Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
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食品和环境样品中沙门氏菌的准总体基因体分析

DOI:
10.3791/58612-v

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06:12 min

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October 25, 2018

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Capitoli

  • 00:04Titolo
  • 00:30Sample Preparation
  • 01:39Immunomagnetic Separation (IMS) and Multiple Displacement Amplification (MDA)
  • 03:37Real-time PCR and Library Preparation for Quasimetagenomic Sequencing
  • 04:52Results: RT-PCR Amplifies DNA from Two Brands of Salmonella Contaminated Chicken Breast
  • 05:19Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们提出了一个协议, 准备从食物和环境微生物群 DNA 样本, 以协同检测和子类型沙门氏菌通过 quasimetagenomic 测序。结合使用培养富集、免疫磁分离 (IMS) 和多重置换扩增 (MDA), 可有效地将沙门氏菌基因组 DNA 从食物和环境样本中浓缩。

Tags

Keywords: SalmonellaQuasi-metagenomic AnalysisFood SampleEnvironmental SamplePathogen DetectionGenetic FingerprintingRV Enrichment BrothCentrifugationAnti-Salmonella BeadsMagnetic SeparationMultiple Displacement Amplification (MDA)

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