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监测小鼠睡眠/觉醒状态期间神经回路的光遗传学操作

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice

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监测小鼠睡眠/觉醒状态期间神经回路的光遗传学操作

DOI:

10.3791/58613-v

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08:58 min

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June 19, 2019

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Shota Kodani*¹, Shingo Soya*², Takeshi Sakurai³

¹Department of Molecular Neuroscience and Integrative Physiology, Faculty of Medicine,Kanazawa University, ²International Institute for Integrative Sleep Medicine (WPI-IIIS),University of Tsukuba, ³Faculty of Medicine,University of Tsukuba

Capitoli

00:04Titolo
00:38Animal Preparation
01:33Virus Injection
02:47Electroencephalogram and Electromyogram (EEG/EMG) Electrode and Optical Fiber Implantation
05:20EEG/EMG Monitoring
07:19Results: Representative Effect of GABA^BNST Stimulation on State Transition in NREM and REM Sleep
08:09Conclusion

Summary

Traduzione automatica

在这里,我们描述了在监测小鼠睡眠/觉醒状态期间对特定类型神经元的光遗传学操作方法,并举例说,我们最近对纹状体末期骨细胞核的床核进行了实验。

Tags

Keywords: Optogenetic Manipulation Neural Circuits Sleep/wakefulness Mice Neuro Circuit Sleep Wakefulness Regulation Surface EEG Anesthetized Mouse Stereotactic Apparatus Adeno-associated Virus Vector Microinjection Bed Nucleus Of The Stria Terminalis (BNST)Electroencephalogram (EEG)Electromyogram (EMG)

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