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Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques
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Capitoli
- 00:04Titolo
- 00:43Generate Samples for Imaging
- 02:13Set up Microscope for Imaging
- 03:57Acquire FRET-FRAP Data
- 06:53Results: Vinculin Force-Sensitive Dynamics at the Focal Adhesion
- 07:52Conclusion
Here, we present a protocol for the simultaneous use of Förster resonance energy transfer-based tension sensors to measure protein load and fluorescence recovery after photobleaching to measure protein dynamics enabling the measurement of force-sensitive protein dynamics within living cells.
