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精子膜评价的荧光技术

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Fluorimetric Techniques for the Assessment of Sperm Membranes

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精子膜评价的荧光技术

DOI:

10.3791/58622-v

•

08:58 min

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November 28, 2018

•

Alisa Komsky-Elbaz², Zvi Roth²

¹Animal Sperm Research Center, Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem, ²Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem

Capitoli

00:04Titolo
00:55Sperm Sample Preparation
02:03Technique #1: Simultaneous Assessment of Sperm Membranes Using Multiple Flourescent Probes
04:34Technique #2: Assessment of Sperm Membranes with Ready-to-use Kits and Flow Cytometry
06:58Results: Evaluation of Semen Quality
08:16Conclusion

Summary

Traduzione automatica

在这里, 我们提出的方法来评估精子细胞膜的完整性, 一个细胞特征与精子受精能力。我们描述了精子膜荧光测量评估的三种技术: 与特定荧光探针同时染色, 荧光显微镜, 和先进的精子专用流式细胞仪。还介绍了合并这些方法的实例。

Tags

Fluorimetric Techniques Sperm Membranes Fluorescent Probes Fluorescence Microscopy Flow Cytometry Sperm Fertilization Potential Sperm Plasma Membrane Acrosome Membrane Mitochondrial Membrane Potential NKM Buffer DAPI FITC-PSA JC-1 PI Epifluorescence Microscopy

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