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Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method

DOI:
10.3791/58636-v

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12:03 min

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April 18, 2019

•

,
1Department of Pathology,Beth Israel Deaconess Medical Center, 2Division of Infectious Diseases,Boston Children’s Hospital, 3Harvard Medical School

Capitoli

  • 00:04Titolo
  • 01:05Inkjet Printer-assisted Automated Checkerboard Array Synergy
  • 03:52Time-kill Synergy Testing: Preparation
  • 08:09Time-kill Synergy Testing: Plating
  • 09:20Deciphering Checkerboard Array Results
  • 11:30Conclusion

Summary

Traduzione automatica

Traduzione automatica

Antimicrobial synergy testing is used to evaluate the effect of two or more antibiotics used in combination and is typically performed by one of two methods: the checkerboard array or the time-kill assay. Here, we present an automated, inkjet printer-assisted checkerboard array synergy technique and a classic time-kill synergy study.

Tags

Antimicrobial Synergy TestingInkjet Printer-assisted Automated Checkerboard ArrayManual Time-kill MethodAntibiotic ResistanceCombination Drug TestingColistinMinocyclineBacterial ConcentrationMcFarland TurbidityCation-adjusted Mueller-Hinton BrothColony-forming Units

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