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Etablering af Dual humaniseret TK-NOG muse model for HIV-associeret lever patogenesen
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis

Etablering af Dual humaniseret TK-NOG muse model for HIV-associeret lever patogenesen

DOI:
10.3791/58645-v

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00:10 min

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September 11, 2019

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, , , ,
1Department of Pharmacology and Experimental Neuroscience,University of Nebraska Medical Center, 2Department of Pathology and Microbiology,University of Nebraska Medical Center

Capitoli

  • 00:04Titolo
  • 00:47Processing of Umbilical Cord Blood and the Isolation of Human HSPCs
  • 04:25Preparation of Human Hepatocytes for Transplantation
  • 05:10Preparation for the Surgery and Instrasplenic Transplantation of Human HSPCs and Hepatocytes in Mice
  • 06:50HIV Infection of TK-NOG Mice and Its Effect on the Human Liver and Immune System
  • 07:48Results: Analysis of the Dual Humanized TK-NOG Mouse Model
  • 09:25Conclusion

Summary

Traduzione automatica

Traduzione automatica

Denne protokol giver en pålidelig metode til at etablere humaniserede mus med både menneskelige immunsystem og leverceller. Dual rekonstitueret immundefekt mus opnået via intrasplenisk injektion af humane hepatocytter og CD34+ hæmatopoietiske stamceller er modtagelige for human immuninsufficiens virus-1 infektion og rekapitulere leverskader som observeret i HIV-inficerede patienter.

Tags

Keywords: HIVLiver PathogenesisHumanized Mouse ModelHematopoietic Stem CellsHepatocytesUmbilical Cord BloodCD34 Positive CellsCell SeparationMagnetic Activated Cell Sorting (MACS)Cell CountingCell CultureTransplantation

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