Rivista
/
Medicina
/
Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation
JoVE Journal
Medicina
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Medicina
Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation

Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation

DOI:
10.3791/58647-v

•

07:13 min

•

January 07, 2019

•

, ,
1Department of Pharmacology, Faculty of Dentistry,Mahidol University, 2Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases,National Institutes of Health

Capitoli

  • 00:04Titolo
  • 00:45Blood Sample Preparation and Deoxygenation
  • 02:52Red Blood Cell (RBC) Nitrite Reduction and Vasodilator-stimulated Phosphoprotein (VASP) Western Blotting
  • 04:39Results: Representative Platelet-based Nitric Oxide (NO) Detection
  • 06:18Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present a protocol to address the potential use of platelets as a highly sensitive nitric oxide sensor in blood. It describes initial platelet preparation and the use of nitrite and deoxygenated red blood cells as nitric oxide generators.

Tags

Keywords: PlateletNitric OxideVASP PhosphorylationBloodIn VitroIn VivoP-VASPSensorCentrifugationPlatelet-rich PlasmaTyrode BufferRed Blood CellsDeoxygenationNitrite Reduction

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/58647?article_type=v

Analytical Techniques for Assaying Nitric Oxide Bioactivity
check_url/it/58647?article_type=v

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)
check_url/it/58647?article_type=v

Cerenkov Luminescence Imaging (CLI) for Cancer Therapy Monitoring
check_url/it/58647?article_type=v

5/6th Nephrectomy in Combination with High Salt Diet and Nitric Oxide Synthase Inhibition to Induce Chronic Kidney Disease in the Lewis Rat
check_url/it/58647?article_type=v

Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle
check_url/it/58647?article_type=v

In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice
check_url/it/58647?article_type=v

Routine Screening Method for Microparticles in Platelet Transfusions
check_url/it/58647?article_type=v

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection
check_url/it/58647?article_type=v

Endotoxin Activity Assay for the Detection of Whole Blood Endotoxemia in Critically Ill Patients
check_url/it/58647?article_type=v

A Novel Inhalation Mask System to Deliver High Concentrations of Nitric Oxide Gas in Spontaneously Breathing Subjects

Read Article