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Isolation of Tissue Extracellular Vesicles from the Liver
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Biochimica
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JoVE Journal Biochimica
Isolation of Tissue Extracellular Vesicles from the Liver

Isolation of Tissue Extracellular Vesicles from the Liver

DOI:
10.3791/58649-v

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00:05 min

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August 21, 2019

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, ,
1Department of Transplantation,Mayo Clinic

Capitoli

  • 00:04Titolo
  • 00:52Liver Cannulation and Perfusion
  • 03:03Extracellular Vesicle (EV) Isolation
  • 04:30Results: Representative Liver EV Size and Concentration by Nanoparticle Tracking Analysis (NTA)
  • 05:10Conclusion

Summary

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This is a protocol to isolate tissue extracellular vesicles (EVs) from the liver. The protocol describes a two-step process involving collagenase perfusion followed by differential ultracentrifugation to isolate liver tissue EVs.

Tags

Keywords: Extracellular VesiclesLiverPortal Vein CannulationHBSS PerfusionCollagenase Digestion

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