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聚乙胺包覆氧化铁纳米粒子作为
载体, 在体外
和
体内向
巨噬细胞输送小干扰 rna
JoVE Journal
Medicina
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JoVE Journal
Medicina
Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages
In Vitro
and
In Vivo
Please note that all translations are automatically generated.
Click here for the English version.
聚乙胺包覆氧化铁纳米粒子作为
载体, 在体外
和
体内向
巨噬细胞输送小干扰 rna
DOI:
10.3791/58660-v
•
09:36 min
•
February 05, 2019
•
Na Jia*
1
,
Haoan Wu*
2
,
Juanli Duan
,
Chunxue Wei
,
Kaixuan Wang
,
Yu Zhang
,
Xiaohua Mao
1
Key Laboratory of Ministry of Education for Developmental Genes & Human Diseases, Institute of Life Sciences and School of Medicine
,
Southeast University
,
2
State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials & Devices, School of Biological Science & Medical Engineering
,
Southeast University
Capitoli
00:04
Titolo
01:00
Polyethyleneimine-Coated Superparamagnetic Iron Oxide Nanoparticle (PEI-SPION) Preparation
03:52
PEI-SPION/small interfering (si) RNA Nanoparticle (NP) Agarose Gel Electrophoresis
05:00
In Vitro RAW264.7 Macrophages Transfection
06:55
Results: Representative Biological Characterization of PEI-SPION and PE-SPION/siRNA NPs
08:51
Conclusion
Summary
Traduzione automatica
English (Original)
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Traduzione automatica
我们描述了一种使用聚乙烯烯胺 (pei) 涂层超顺磁性氧化铁纳米粒子与 sirna 转染巨噬细胞的方法。这些纳米颗粒可以有效地传递 sirna 巨噬细胞在
体外
和
体内
和沉默目标基因表达。
Tags
Polyethyleneimine
Iron Oxide Nanoparticles
SPION
Macrophage
Delivery
SiRNA
In Vitro
In Vivo
Allylic Acid
Dimercapto Acrylic Acid
Triethylamine
PH
Nanoparticle Synthesis
Coating
Inflammation
Cancer
Article
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