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聚乙胺包覆氧化铁纳米粒子作为载体, 在体外和体内向巨噬细胞输送小干扰 rna
JoVE Journal
Medicina
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JoVE Journal Medicina
Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo
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聚乙胺包覆氧化铁纳米粒子作为载体, 在体外和体内向巨噬细胞输送小干扰 rna

DOI:
10.3791/58660-v

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09:36 min

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February 05, 2019

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, , , , , ,
1Key Laboratory of Ministry of Education for Developmental Genes & Human Diseases, Institute of Life Sciences and School of Medicine,Southeast University, 2State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials & Devices, School of Biological Science & Medical Engineering,Southeast University

Capitoli

  • 00:04Titolo
  • 01:00Polyethyleneimine-Coated Superparamagnetic Iron Oxide Nanoparticle (PEI-SPION) Preparation
  • 03:52PEI-SPION/small interfering (si) RNA Nanoparticle (NP) Agarose Gel Electrophoresis
  • 05:00In Vitro RAW264.7 Macrophages Transfection
  • 06:55Results: Representative Biological Characterization of PEI-SPION and PE-SPION/siRNA NPs
  • 08:51Conclusion

Summary

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我们描述了一种使用聚乙烯烯胺 (pei) 涂层超顺磁性氧化铁纳米粒子与 sirna 转染巨噬细胞的方法。这些纳米颗粒可以有效地传递 sirna 巨噬细胞在体外和体内和沉默目标基因表达。

Tags

PolyethyleneimineIron Oxide NanoparticlesSPIONMacrophageDeliverySiRNAIn VitroIn VivoAllylic AcidDimercapto Acrylic AcidTriethylaminePHNanoparticle SynthesisCoatingInflammationCancer

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