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Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

DOI:
10.3791/58664-v

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07:07 min

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January 07, 2019

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1Pulmonary, Immunology and Physiology Laboratory, Department of Pediatrics,Pennsylvania State University College of Medicine, 2Department of Biochemistry and Molecular Biology,Pennsylvania State University College of Medicine

Capitoli

  • 00:04Titolo
  • 00:31Estrous Cycle Stage Assessment, Ozone Exposure, and Lung Collection
  • 02:11RNA Preparation
  • 03:43microRNA (miRNA) Profiling
  • 04:40Results: Representative Functional microRNA Analyses
  • 06:26Conclusion

Summary

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Here we describe a method to assess lung expression of miRNAs that are predicted to regulate inflammatory genes using mice exposed to ozone or filtered air at different stages of the estrous cycle.

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Keywords: MicroRNALungEstrous CycleOzoneMouseInflammationVaginal FluidRNA ExtractionReal-time PCR

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