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Fluorescent coloration à l’argent des protéines dans les Gels de Polyacrylamide
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1Ming Wai Lau Centre for Reparative Medicine,Karolinska Institutet, 2Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science,Hong Kong University of Science and Technology
Capitoli
- 00:04Titolo
- 00:36Preparation of the Gel
- 01:08Fixation of the Gel
- 01:40Preparation of the AgNO3 Solution and Silver Impregnation of the Gel
- 02:41Fluorogenic Development of the Gel
- 04:05Destaining and Imaging
- 04:34Results: Comparison of the Fluorescent Silver Staining to Other Staining Methods
- 05:56Conclusion
Nous décrivons ici un protocole détaillé décrivant un fluorescent nouvelle coloration pour la détection de protéines totales dans les gels de polyacrylamide. Le protocole utilise une sonde de turn-on argent ionique spécifique fluorescence, qui permet de détecter Ag+-complexes de protéine et élimine certaines limitations des taches argent chromogène traditionnels.
