Rivista
/
Biochimica
/
Using High Content Imaging to Quantify Target Engagement in Adherent Cells
JoVE Journal
Biochimica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biochimica
Using High Content Imaging to Quantify Target Engagement in Adherent Cells

Using High Content Imaging to Quantify Target Engagement in Adherent Cells

DOI:
10.3791/58670-v

•

07:23 min

•

November 29, 2018

•

, ,
1Chemical Biology Consortium Sweden, Science for Life Laboratory,Karolinska Institutet, Solna, 2Department of Medical Biochemistry and Biophysics,Karolinska Institutet, Solna, 3Science for Life Laboratory, Department of Oncology-Pathology,Karolinska Institutet, Stockholm

Capitoli

  • 00:04Titolo
  • 00:31Cell Seeding
  • 01:51Compound Treatment and Heat Challenge
  • 03:30Staining, Image Acquisition and Analysis
  • 05:33Results: Representative Target Engagement Quantification by High Content Imaging
  • 06:54Conclusion

Summary

Traduzione automatica

Traduzione automatica

Measurements of drug target engagement are central to effective drug development and chemical probe validation. Here, we detail a protocol for measuring drug-target engagement using high content imaging in a microplate-compatible adaption of the cellular thermal shift assay (CETSA).

Tags

High Content ImagingTarget EngagementAdherent CellsChemical BiologyDrug DiscoveryCell LinesPrimary CulturesCoculturesA-431 CellsTrypsinPlate WashingCompound TreatmentHeat ChallengeWater Bath

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Determination of the Glycogen Content in Cyanobacteria

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)

A Colorimetric Method for Measuring Iron Content in Plants

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator

How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells

Direct Measurement of KDM1A Target Engagement Using Chemoprobe-based Immunoassays

Detection of Total Reactive Oxygen Species in Adherent Cells by 2′,7′-Dichlorodihydrofluorescein Diacetate Staining

Estimation of Plant Biomass Lignin Content using Thioglycolic Acid (TGA)

Förster Resonance Energy Transfer Measurements in Living Plant Cells

Read Article