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补充蛋白C1q和透明质酸在促进细胞粘附中的相互作用评价

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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

Please note that all translations are automatically generated. Click here for the English version.

补充蛋白C1q和透明质酸在促进细胞粘附中的相互作用评价

DOI:

10.3791/58688-v

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06:54 min

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June 15, 2019

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Romana Vidergar*¹, Chiara Agostinis*², Paola Zacchi, Alessandro Mangogna, Fleur Bossi, Fabrizio Zanconati, Marco Confalonieri, Giuseppe Ricci³, Roberta Bulla

¹Department of Life Sciences,University of Trieste, ²Institute for Maternal and Child Health,IRCCS (Istituto di Ricovero e Cura a Carattere Scientifico) Burlo Garofolo, ³Department of Medical, Surgical and Health Science,University of Trieste

Capitoli

00:00Titolo
00:59HA and C1q Coating Procedure
02:45Cells Labelling with Fast Dil
04:04Cells Adhesion on HA/C1q Matrices
05:32Risultati
06:02Conclusion

Summary

Traduzione automatica

补体组分C1q是一种在组织微环境中高度表达的亲炎分子,可与细胞外基质相互作用。在这里,我们描述了一种测试C1q与透明质酸结合如何影响细胞粘附的方法。

Tags

Keywords: C1q Hyaluronic Acid Cell Adhesion Extracellular Matrix Innate Immune System Tumor Microenvironment Placenta Microenvironment Cell Division Binary Cells Coating Procedure Enzyme-linked Immunoassay Fast DiI Cell Labeling

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