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低氧环境下人巨噬细胞极化的蛋白质组学分析

JoVE Journal
Biochimica

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JoVE Journal Biochimica

Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment

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低氧环境下人巨噬细胞极化的蛋白质组学分析

DOI:

10.3791/58727-v

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10:15 min

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January 07, 2019

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Magali Court², Marie Malier², Arnaud Millet^2,3

¹Team Mechanobiology, Immunity and Cancer, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309, ²Université Grenoble Alpes, ³Pôle Recherche,Centre Hospitalier Universitaire des Alpes

Capitoli

00:04Titolo
00:40Isolation of Peripheral Blood Mononuclear Cells (PBMCs) from Leukoreduction System Chamber (LRSC)
02:34Magnetic Labeling and Isolation of CD14+ Cells (Monocytes)
04:32Lysis and In-Gel Digestion
07:54Results: Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
09:17Conclusion

Summary

Traduzione automatica

我们提出了一个协议, 以获得蛋白质组的特征的人类巨噬细胞, 并应用于确定低氧环境对巨噬细胞极化的影响。

Tags

Keywords: Proteomic Analysis Human Macrophage Polarization Low Oxygen Environment Label-free Quantification PBMC Isolation CD14+ Monocyte Isolation Macrophage Differentiation

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