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Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

DOI:
10.3791/58731-v

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09:16 min

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January 07, 2019

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, , , ,
1Department of Cardiovascular Surgery, Medical Faculty,Heinrich-Heine-University

Capitoli

  • 00:04Titolo
  • 00:36Human Whole Blood Extracellular Vesicle (EV) Isolation
  • 01:55Sample Staining
  • 03:16Sample Analysis
  • 06:41Results: Representative Results of Exosome Characterization
  • 08:21Conclusion

Summary

Traduzione automatica

Traduzione automatica

In this protocol, we describe the complete workflow for rapid isolation of extracellular vesicles from human whole blood and characterization of specific markers by fluorescence-based nanoparticle-tracking analysis. The presented results show a high level of reproducibility and can be adjusted to cell culture supernatants.

Tags

Extracellular VesiclesNanoparticle-tracking AnalysisRapid IsolationFluorescence-based CharacterizationHuman BloodCell Membrane StainingAntibody Labeling

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