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Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor
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Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor

DOI:
10.3791/58739-v

•

08:53 min

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January 12, 2019

•

, , ,
1Sainsbury Laboratory,University of Cambridge

Capitoli

  • 00:04Titolo
  • 00:47Plant Growth and Sample Preparation
  • 02:09Gibberellin4 (GA4) Treatment
  • 03:45Tissue of Interest Time Course Setup
  • 05:093-D Image Analysis
  • 06:36Results: Representative Endogenous and Exogenous GA Gradients in Arabidopsis Roots and Dark-grown Hypocotyls
  • 07:58Conclusion

Summary

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Gibberellin Perception Sensor 1 (GPS1) is the first Förster resonance energy transfer-based biosensor for measuring the cellular levels of gibberellin phytohormones with a high spatiotemporal resolution. This protocol reports on the method to visualize and quantify cellular gibberellin levels using the genetically encoded nlsGPS1 biosensor in Arabidopsis hypocotyls and root tips.

Tags

NlsGPS1FRETArabidopsisPhytohormoneGibberellinCellular ImagingBiosensorPlant DevelopmentRatiometric ImagingMurashige And Skoog AgarStratificationGrowth ChamberHypocotylSteady State MeasurementsGA4Vacuum GreaseMicroscope SlideCover Slip

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