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荧光蛋白对酵母中多谷氨酰胺毒性测定的融合伙伴的影响
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Biologia
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JoVE Journal Biologia
Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast
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荧光蛋白对酵母中多谷氨酰胺毒性测定的融合伙伴的影响

DOI:
10.3791/58748-v

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09:23 min

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November 28, 2018

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, , , ,
1Department of Anatomy and Cell Biology,University of Western Ontario, 2Department of Pathology and Laboratory Medicine,University of Western Ontario

Capitoli

  • 00:04Titolo
  • 00:40Preparing Cell Cultures
  • 02:25Spotting Assay
  • 03:36Quantification of Cell Growth in Liquid Culture
  • 04:49Fluorescent Microscopy
  • 05:32Dot Blot
  • 07:26Results: Effects of Fluorescent Proteins on PolyQ Toxicity and Aggregation in Yeast
  • 08:50Conclusion

Summary

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本文介绍了评估荧光蛋白对错误折叠的多谷氨酰胺扩张的聚集和毒性的影响的协议, 以便在荧光记者的背景下快速评估一种新的无特征荧光蛋白。

Tags

Fluorescent ProteinsFusion PartnersPolyglutamine ToxicityYeastHTT Exon One25Q72QMonomeric Superfolder GFPGAL1 PromoterPolyQ FusionsOptical Density

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