/

Immunologia e infezioni

/

小鼠边缘区 b 细胞剪切流诱导迁移的体外分析

JoVE Journal
Immunologia e infezioni

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Immunologia e infezioni

Analysis of Shear Flow-induced Migration of Murine Marginal Zone B Cells In Vitro

Please note that all translations are automatically generated. Click here for the English version.

小鼠边缘区 b 细胞剪切流诱导迁移的体外分析

DOI:

10.3791/58759-v

•

08:31 min

•

November 26, 2018

•

Kerry Tedford, Laura Tech, Michael Steiner, Mark Korthals, Klaus-Dieter Fischer

¹Institute of Biochemistry and Cell Biology,OVGU University of Magdeburg

Capitoli

00:04Titolo
00:43Preparing the 6-chambered Slides for the Flow Experiment
01:49Fluidics Unit Preparation
03:07Cell Loading
04:04Attaching the Fluidics Unit and Imaging the Cells
05:28Migration Track Analysis
07:02Results: Quantifying Marginal Zone B-cell Shuttling
08:00Conclusion

Summary

Traduzione automatica

边缘区 b 细胞 (mzb) 通过调整其迁移路径的方向来响应剪切流的力。该协议演示了如何使用流体单元、泵、显微镜成像系统和自由软件记录和分析迁移。

Tags

Keywords: Shear Flow Marginal Zone B Cells Migration Assay ICAM-1 Leukocytes Cytoskeleton Integrin Ligands Chemokines Activated T Cells Fluid Pump Migration Buffer Shear Stress

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

激活的B细胞CD40的小鼠模型

迁移人T淋巴细胞分离，培养和分析<em在体外</em

纯净小鼠的B细胞的诱导和评估类开关重组

流式细胞仪分析小鼠主动脉内流的免疫细胞

在体外对HIV - 1核心脱壳

小鼠牙龈细胞的分离，处理和分析

在小鼠B淋巴细胞染色体畸变所PNA-FISH快速分析

B组的小鼠模型<em>链球菌</em>阴道定植

检测和罕见富集抗原特异性B细胞的表型和功能分析

一种新的体外创面愈合试验评价细胞迁移

Read Article