Rivista
/
Immunologia e infezioni
/
Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model

Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model

DOI:
10.3791/58768-v

•

06:15 min

•

December 20, 2018

•

, , ,
1Institute of Basic Medical Sciences, College of Medicine,National Cheng Kung University, 2Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine,National Cheng Kung University, 3Department of Biochemistry and Molecular Biology, College of Medicine,National Cheng Kung University, 4Department of Microbiology and Immunology, College of Medicine,National Cheng Kung University

Capitoli

  • 00:04Titolo
  • 00:37Synchronization of C. elegans
  • 02:24C. elegans Survival Assay with Aeromonas
  • 03:22C. elegans Liquid Toxicity Assay with Aeromonas
  • 04:14C. elegans Muscle Necrosis Assay with Aeromonas
  • 04:57Results: Diversity in Survival Rates of C. elegans Infected with Aeromonas spp. Consistent with Clinical Observations
  • 05:45Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we introduce three different experiments to study Aeromonas infection in C. elegans. Using these convenient methods, it is easy to evaluate the toxicity among and within Aeromonas species.

Tags

AeromonasCaenorhabditis ElegansVirulencePathogenesisHost-pathogen InteractionToxicityPathogenicityL1 WormsL4 WormsNGM PlatesE. Coli OP50Bacterial BrothOD 600 Absorbance

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

In vitro Biofilm Formation in an 8-well Chamber Slide

Using Luciferase to Image Bacterial Infections in Mice

The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

A Protocol to Infect Caenorhabditis elegans with Salmonella typhimurium

Influenza A Virus Studies in a Mouse Model of Infection

The Madagascar Hissing Cockroach as an Alternative Non-mammalian Animal Model to Investigate Virulence, Pathogenesis, and Drug Efficacy

Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

Read Article